Coding

Part:BBa_K4830018:Design

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-11)


MMTV RT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.

Source

NCBI ACCESSION: AAC82556

References

Pr160 [mouse mammary tumor virus] - protein - NCBI (no date) National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/AAC82556.1?report=genbank&log%24=protalign&blast_rank=1&RID=JD4PC8M2013 (Accessed: 12 October 2023).